Some Basic Facts to Learn about Protein Purification- Here is a Simple Guide
In many research works, protein purification is a preliminary step as it is required for producing a purified protein that is used for analyzing individual proteins separately and understanding their functions, structures and interactions. But, while studying a protein of interest, you need to overcome a series of steps that are involved with the process of protein purification. These steps help in isolating one or multiple proteins from a complex mixture and provide you with the protein that you need. Purified protein serves as a biochemical reagent that has a great value in the production of purified growth factors or hormones, proteases, DNA polymerases, reverse transcriptases, ligases, phosphatases, or antibodies. The Purpose of the Protein Purification Process: Protein purification is used for separating protein and non-protein parts in a mixture which can be cells, tissues or an organism. After the separation of the protein and non-proteins, the researchers can isolate the...