Basic Needs for Biological Research- BIO BASIC

To study modified oligonucleotides chemical synthesis can be the most powerful approach. In order to study the biological structure stability, and biological activities of RNAs. Modified RNAs have special properties that can enhance the drug properties of RNA oligomers. Nucleobase residues are the important sites of RNA modification or functionalization. Recently, the use of modified oligoribonucleotides has significantly increased in the field of molecular biology, biochemistry, and medicines. Improved mass spectrometry approaches and highly sensitive chemical screens in cellular RNA isolation are still playing an important role in extending the list of new base-modifying units. The precious data on RNA structure and cellular activities can also be extracted from structural, biophysical, and biochemical studies. Nowadays, b oth DNA and RNA oligos can be customized and are available in various formats including custom RNA oligos in tubes, custom RNA oligos in plates, rapid kits, and RN...

Oligonucleotides, often known as oligos, are short strands of synthetic DNA or RNA that the user has constructed. Synthesis is the method used to create custom oligos (or more specifically, solid-phase chemical synthesis). The nucleic acids Cytosine (C), Guanine (G), Adenine (A), Thymine (T) for DNA, and Uracil (U) for RNA are sequentially added to a developing chain of nucleotides in oligo synthesis . The nucleic acids must be constructed on their phosphate backbone to form the chain, which is then produced in the 3' to 5' orientation. After synthesis, the oligo is usually desalted (all salts utilized during synthesis are removed) and/or purified (removes non-full-length material). In the polymerase chain reaction(PCR), oligos are routinely utilized. PCR is a technique for producing thousands or millions of copies of a single strand of DNA for use in other downstream applications (i.e. cloning, sequencing, etc.). Primers are oligos that are between 20 and 35 bases long and ar...