Top Things You Need To Know About Culture Media

Culture media is a nutrient-containing gel or liquid used to cultivate bacteria or microorganisms in the laboratory. This medium facilitates the reproduction, growth, and counting of microorganisms and their survival. In the laboratory, culture media are used for quality control testing of non-sterile raw materials and finished products and microbiological contamination (sterility) testing in applications including hygiene monitoring, sterilization process validation, and determining the efficacy of preservatives and antimicrobial agents.


Contents Of Culture Media:

Growing microorganisms in the laboratory necessitate simulating the organisms' natural habitat or environment, which can be accomplished by creating culture media that fulfills their needs. The primary medium includes a carbon and energy source, a nitrogen supply, growth agents, and trace elements. Standard media components include peptone, agar, water, casein hydrolysate, malt extract, meat extract, and yeast extract. In addition, the medium's pH must be appropriately adjusted.



Types Of Culture Media:

Scientists have devised numerous culture mediums based on the microbial species to be cultivated. The following are the three primary classifications of culture media based on compositions- 

  • Solid media: A long, unbranched polysaccharide chain is added to agar at a concentration of 1.5% to 2.0%. In laboratories, 1.3% agar is widely used to make solid media. In place of agar, alternative inert solidifying agents, such as gellan gum, are utilized. Solid media are used to cultivate microorganisms in their physical form, to prepare pure bacterial cultures, and to isolate bacteria to study colony properties.

  • Semisolid media: This media has a 0.2-0.5% agar concentration and appears soft, jelly-like substance due to the lower agar content. It is mainly used to examine the movement of microorganisms, differentiate between motile and non-motile bacterial strains, and produce microaerophilic bacteria — bacteria appear as a thick line on this medium.

  • Liquid media: These media include no residues of hardening agents, such as agar or gelatin, and they support the rapid growth of bacterial colonies. The medium is utilized for microorganism proliferation and fermentation research.


Final Words:

You can buy a large variety of culture media at competitive prices in Canada from the online store of Bio Basic. In addition, this reputed biotechnology company provides free shipping in Canada for orders over $299.

So, don't wait anymore! Explore the wide range of collections of Bio Basic now.

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