What Else to Know About Custom Antibody

Antibodies are generated due to the adaptive immune system. This happens in response to invading pathogens. The antibody offers specific lock and key recognition as it offends bacteria, viruses, or other molecules. They are collectively called antigens.

Antibodies are proteins. These are folded polypeptides or strands of amino acids. They have antigen recognition sites. They are generated by B-cells of the adaptive immune system.

When an antibody is required for a particular antigen, but it isn’t readily available from a supplier, you may possibly have antibodies made via custom antibody production. A 90% purified sample of antigen is all that is required to get a custom antibody generated and harvested against that antigen. If you can not get a sample of antigen, probably you will have custom peptides or proteins synthesized and antibodies created against them. Both polyclonal and monoclonal antibodies are possibly to be produced by custom antibody production protocols.



Custom monoclonal antibody production

Custom monoclonal antibody production needs 6 months from start to finish. There are 5 steps:

  • antigen preparation
  • hybridoma development immunizations
  • hybridoma development fusions
  • subcloning
  • monoclonal production and purification

Antigen preparation: 

The customer may provide highly pure protein or peptide. The company could also present a design and synthesis of a custom peptide or produce a protein.

Immunization: 

Animals get immunized and their serum is tested for antibody production

Fusion: 

The animals with the best immune response to the antigen get further boosts of the antigen before harvesting their spleens for hybridoma fusion. A hybridoma is a cell line of immortal B cells and myeloma cancer cells. These cells have good longevity and reproductivity. 

Subcloning: 

The cell line is cloned till it is homogeneous and constantly secreting antibodies.

Bio Basic is an online portal where you can place your research necessary orders for custom antibody production. They offer antibody synthesis packages. These packages are structured to give you the freedom to synthesize (from cDNA, peptide or protein) an antibody.

 

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