What to Know About Gene Synthesis?

Gene synthesis is a well-known process among researchers that enables them to specify a desired sequence and custom-build the gene directly. Gene synthesis is a straightforward, faster, and cost-effective process than other alternative methods of molecular cloning and mutagenesis. This process involves multiple steps that include cloning strategy design, primer synthesis, PCR, gel extraction, bacteria transformation, and other complex steps, gene synthesis can give you a faster and more accurate outcome.

Here are the steps of gene synthesis process in brief-

  1. Oligonucleotide synthesis
  2. Amplification: PCR
  3. Fragment Assembly
  4. Fragment Sequencing
  5. Correcting Errors: Fragments
  6. Vector Insertion
  7. Selecting Clones
  8. Sequencing Gene: First Round
  9. Correcting Errors: Gene
  10. Sequencing Gene: Final Round

Key Advantages of Gene Synthesis

  • This process can be used for a short period of synthesis cycle and 100% correct sequence.
  • It delivers excellent flexibility in modifying restriction sites and gene sequences to execute subsequent cloning experiments.
  • It is a perfect process for hard-to-obtain genes that are non-existent in nature.
  • Synthesized genes produced by the gene synthesis process are optimized in codons.

If you are planning to get high-quality gene synthesis services to produce your desired sequence for your research, contact a life science research support company near you and get their assistance for your subcloning projects. These companies can help you with their custom genes and gene synthesis services for small custom projects as well as large-scale projects. Browse the website of reputable life science research support companies or call the suppliers to satisfy all your queries.


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